Journal: Cell proliferation
Article Title: Deficiency of Aging-Related Gene Chitinase-Like 4 Impairs Olfactory Epithelium Homeostasis.
doi: 10.1111/cpr.70055
Figure Lengend Snippet: FIGURE 3 | Chil4 regulates proliferation and neuronal generation in OE organoids. (A–F) Confocal images of OMP+, IL33+, Ki67+, PGP9.5+ cells in young organoids infected with control AAV-mCherry or AAV-Chil4-mCherry (A, C, E), and in aged organoids infected with control AAV-GFP or AAV-shChli4-GFP (B, D, F). (G–I) Quantification of OMP+ (G), IL33+ (H), Ki67+ (I) cells in young organoids infected with control AAV-mCherry or AAV-Chil4-mCherry, and in aged organoids infected with control AAV-GFP or AAV-shChli4-GFP. n = 6, 4, 8, 5 preparations in AAV-Ctrl-mCherry, AAV-Chil4-mCherry, AAV-Ctrl-GFP, AAV-shChil4-GFP group in (E), n = 8, 7, 7, 7 preparations in (F), n = 8, 12, 9, 8 preparations in (G). (J–M) Confocal images and quantification of Ki67+ (J), OMP+ (K), Tuj1+ (L), Trp63+ (M) cells in the WT and Chil4−/− OE organoids. n = 13 and 12 preparations for WT and Chil4−/− organoids in (J), n = 10 and 9 preparations in (K), n = 12 and 11 preparations in (L), n = 10 and 9 preparations in (M). Organoids in each group were derived from three animals. The statistical significance was determined by un- paired t test. ns, not significant, *p < 0.05, ***p < 0.001 and ****p < 0.0001. Scale bars: 10 μm.
Article Snippet: The primary antibodies used were as follows: rabbit anti- Krt14 (#10143–1- AP, Proteintech), rabbit anti- Sox2 (#AF- 2018, R&D Systems), goat anti- Icam1 (#AF796, R&D Systems), rabbit anti- Chil4 (#ab192029, Abcam), rabbit anti- OMP (#ab183947, Abcam), mouse anti- Tuj1 (#ab78078, Abcam), rabbit antiGAP43 (#ab75810, Abcam), rabbit anti- NeuroD1 (#ab205300, Abcam), mouse anti- Ki67 (#550609, BD Biosciences), goat anti- IL33 (#AF- 3626, R&D Systems), mouse anti- Cdk1 (#sc- 54, SantaCruz), goat anti- Iba1 (#ab5076, Abcam), rabbit anti- F4/80 (#GB113373, Servicebio), rabbit anti- MPO (#ab208670, Abcam), rat anti- CD44 (#550538, BD Biosciences), rabbit anti- Dclk1 (#21699- 1- AP, Proteintech).
Techniques: Infection, Control, Derivative Assay